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Hairy root induction and polysaccharide production of

Hairy root induction and polysaccharide production of

Hairy root induction and polysaccharide production of ...

2016-4-7  A reliable and efficient hairy root induction system for the important medicinal plant Callerya speciosa was established by optimizing several parameters that affected the efficiency of Agrobacterium rhizogenes-mediated transformations. Four strains, A4, LBA9402, R1601 and C58C1, and four types of explants, hypocotyls, cotyledons, leaves and excised stems, were examined and the highest rate of ...

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Hairy root induction and polysaccharide production of ...

Download Citation Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ. A reliable and efficient hairy root induction system for the important ...

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Hairy root induction and Farnesiferol B production of ...

2019-10-22  Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ. Plant Cell Tissue Organ Cult. 2016; 126:177–186. [Google Scholar] Yonemitsu H, Shimomura K, Satake M, Mochida S, Tanaka M, Endo T, Kaji A. Lobeline production by hairy root culture of Lobelia inflata L. Plant Cell Rep. 1990; 9:307–310.

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Astragaloside IV and polysaccharide production by hairy ...

Time course of growth, astragaloside IV and polysaccharide production by hairy roots of A. membranaceus cultured in 250 ml conical flasks. Growth of A. membranaceus hairy roots is

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Hairy root induction and Farnesiferol B production of ...

Hairy root induction and Farnesiferol B production of endemic medicinal plant Ferula pseudalliacea. Khazaei A(1), Bahramnejad B(1), Mozafari AA(2), Dastan D(3)(4), Mohammadi S(1). Author information: (1)1Department of Agronomy and Plant Breeding, University of Kurdistan, 416, Sanandaj, Iran.

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Hairy root induction and Farnesiferol B production of ...

2019-10-22  This is the first report of hairy root induction in this species and also production of farnesiferol B in its hairy roots. This system provides an efficient tool for hairy root production of F. pseudalliacea and is useful for commercial production of important secondary metabolites such as farnesiferol B under in vitro conditions.

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Promotion of Salvia miltiorrhiza hairy root growth and ...

2010-9-1  This study was to examine the effects of polysaccharides from a plant growth-promoting rhizobacterium (PGPR) Bacillus cereus on the growth and tanshinone production of Salvia miltiorrhiza hairy roots. A polysaccharide fraction designated BPS was isolated from the hot water extract of B. cereus cells by ethanol precipitation. BPS applied to the root culture at 100–400 mg l −1 a few days ...

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Astragaloside IV and polysaccharide production by hairy ...

Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g −1 astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l −1, astragaloside IV 0.9 mg g −1).Polysaccharide yields were similar amongst the different ...

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Hairy root induction and Farnesiferol B production of ...

Hairy root culture is known for its genetic and physiochemical stability and increased production of secondary metabolites as compared to cell suspension culture and with the use of low frequency ...

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Hairy Root Culture: History, Formulation and Application

2021-8-12  Hairy root culture of Chinese medicinal plant tri-chosanthes kirilowii have been seen producing trichosanthin, a ribosome inactivating proteins, can inhibit multiplication of the Human Immuno Deficiency (HIV) THE AIDS virus. In recent year, hairy root culture has emerged as useful to study the biology and engineering of plant chemical production.

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Hairy root induction and benzylisoquinoline alkaloid ...

Consequently, this study demonstrated that the hairy root system has further potential for bioengineering and sustainable production of sanguinarine on a commercial scale. To the best of our knowledge, this is the first efficient protocol reported for the establishment of hairy root

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Hairy Root Induction in Helicteres isora L. and Production ...

DOI: 10.1007/s13659-014-0011-9 Corpus ID: 244250. Hairy Root Induction in Helicteres isora L. and Production of Diosgenin in Hairy Roots @article{Kumar2014HairyRI, title={Hairy Root Induction in Helicteres isora L. and Production of Diosgenin in Hairy Roots}, author={V. Kumar and Dnyanada Desai and Varsha Shriram}, journal={Natural Products and Bioprospecting}, year={2014}, volume={4}, pages ...

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Hairy root induction and Farnesiferol B production of ...

Hairy root induction and Farnesiferol B production of endemic medicinal plant Ferula pseudalliacea. Khazaei A(1), Bahramnejad B(1), Mozafari AA(2), Dastan D(3)(4), Mohammadi S(1). Author information: (1)1Department of Agronomy and Plant Breeding, University of Kurdistan, 416, Sanandaj, Iran.

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Astragaloside IV and polysaccharide production by hairy ...

2004-10-6  Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g−1 astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l−1, astragaloside IV 0.9 mg g−1). Polysaccharide yields were similar amongst the different ...

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Hairy Root Induction in Helicteres isora L. and Production ...

2017-4-5  2.3 Hairy Root Induction and Proliferation For hairy root induction process, selection of A. rhizogenes strains, type of explants and growth medium compositions are crucial components. In present investigation, various parameters were optimized for efficient Agrobacterium-mediated transformation. Two A. rhizogenes strains,

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A comparative study of Hairy Root Culture induction ...

2017-7-18  Hairy root culture, Agrobacterium rhizogenes, Aegle marmelos, Boerhavia diffusa, Datura innoxia, Solanum xanthocarpum, Hairy Root Culture is the new route for large scale secondary metabolite production because of their fast and plagiotropic growth, genetic and biochemical stability.

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Efficient production of flavonoids in Fagopyrum tataricum ...

The exogenous yeast polysaccharide (YPS) elicitor notably stimulated the functional metabolites production in F. tataricum hairy root cultures, and the stimulation effect was concentration-dependent. Combination with the YPS elicitation (200 mg/L) and medium renewal process, the maximal flavonoids y

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Agrobacterium rhizogenes mediated hairy root induction

2017-8-29  An efficient protocol for hairy root induction in Berberis aristata DC. was established using two different strains of Agrobacterium rhizogenes, MTCC 532 and 2364 from IMTECH (Institute of Microbial Technology), Chandigarh, India. The strain 532 was more effective than strain 2364 in hairy root induction and in vitro grown callus

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Efficient production of flavonoids in Fagopyrum tataricum ...

2016-11-6  Efficient production of flavonoids in Fagopyrum tataricum hairy root cultures with yeast polysaccharide elicitation and medium renewal process.[J]. Zhao J L, Zou L, Zhang C Q, et al.Pharmacognosy Magazine, 2014, 10(39):234-40.

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Effects of the rol C Gene on Hairy Root: Induction ...

2019-5-26  Two series of Atropa belladonna hairy root lines were obtained, the first one transformed via Agrobacterium tumefaciens harboring rol C and npt II genes, and the other transformed with rol ABC and npt II genes. Thirteen hairy root lines were obtained and selected on hormone-free medium. The transformation was confirmed by PCR analysis, and these root lines were first examinated for their ...

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Astragaloside IV and polysaccharide production by hairy ...

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Hairy roots of Astragalus membranaceus were grown in bioreactors up to 30 l for 20 d. Cultures from a 30 l airlift bioreactor gave 11.5 g l dry wt with 1.4 mg g−1 astragaloside IV, similar to cultures from 250 ml and 1 l flasks, but greater than yields from a 10 l bioreactor (dry wt 9.4 g l−1, astragaloside IV 0.9 ...

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A comparative study of Hairy Root Culture induction ...

2017-7-18  Hairy root culture, Agrobacterium rhizogenes, Aegle marmelos, Boerhavia diffusa, Datura innoxia, Solanum xanthocarpum, Hairy Root Culture is the new route for large scale secondary metabolite production because of their fast and plagiotropic growth, genetic and biochemical stability.

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The High Efficient Induction of Hairy Roots of Astragalus ...

The most suitable A 600 value of A. rhizogenes R1601 for the infection was 1.5, which could achieve the maximum induction rate of hairy roots from leaves as 94.9%. The PCR identification showed that the hairy roots were induced by A. rhizogenes R1601. The biomasses of different hairy root strains had significant difference.

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Effects of the rol C Gene on Hairy Root: Induction ...

2019-5-26  Two series of Atropa belladonna hairy root lines were obtained, the first one transformed via Agrobacterium tumefaciens harboring rol C and npt II genes, and the other transformed with rol ABC and npt II genes. Thirteen hairy root lines were obtained and selected on hormone-free medium. The transformation was confirmed by PCR analysis, and these root lines were first examinated for their ...

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Influences of various factors on hairy root induction in ...

Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A. rhizogenes, rosmarinic acid content and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of A. foeniculum was established by inoculation of the 1 ...

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Plant hairy roots enable high throughput identification of ...

2020-11-16  The transformation efficiency of the hairy root induction, defined by the percentage of GFP-positive hairy roots among all roots generated, was ~70–90% in

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Establishment of hairy root cultures and analysis of ...

2021-7-30  In this research, our results demonstrated the induction of transgenic hairy root lines of T. paniculatum, screening, and selection for saponin production. Two Agrobacterium rhizogenes strains, ATCC 15843 and LBA 9402/12 were used to induce hairy roots following infection of leaf explants from in vitro and ex vitro-grown plants.

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Response of Different Agrobacterium Rhizogenes Strains for ...

Response of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache Rugosa Woo Tae Park 1, Thanislas Bastin Baskar 1, Sun Kyung Yeo 1, Nam Il Park 2, Jong Seok Park 1 and Sangun Park 1. 1 Chungnam National University, Korea; 2 Gangneung-Wonju National University, Korea

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Transgenic Hairy Root Induction of Lens culinaris

2020-8-10  hairy root induction (Figure1b). Inoculation was performed by excising the existing roots in the hypocotyl region using a sterile scalpel and dipping the wounded root tip into the bacterial cells. Inoculated lentil seedlings were co-cultivated with A. rhizogenes for 3 days (23 C, 16/8 h light/darkness)

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Genetic transfection, hairy root induction and solasodine ...

An efficient genetic transfection technique has been established using A4 strain of Agrobacterium rhizogenes for the first time in a medicinally valuable plant Solanum erianthum D. Don. The explants were randomly pricked with sterile needle, inoculated with bacterial suspension. The infected leaves were then washed and transferred to MS basal medium fortified with cefotaxime for hairy root ...

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